Real-time monitoring of rhamnose induction effect on the expression of mpt64 gene fused with pelB signal peptide in Escherichia coli BL21 (DE3)

In this research, Escherichia coli BL21 (DE3) harboring an expression vector constructed with a rhamnose-inducible promoter and a pelB signal peptide was used as a host cell to produce MPT64 protein.The objective of this research was to figure out the optimum time of mpt64 gene expression through real-time monitoring of MPT64 protein production and

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Thermomechanical analysis for the theoretical optimization of W/Cu monoblocks with functionally graded interlayer

In fusion devices, plasma-facing components (PFCs) play a critical role in withstanding severe thermal conditions resulting from cyclic high heat flux (HHF) loads.The International Thermonuclear Experimental Reactor (ITER) and next-generation fusion devices are expected to employ actively cooled tungsten/copper (W/Cu) monoblocks as divertor targets

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